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R128
Sigma
Monoclonal Anti-Ryanodine Receptor antibody produced in mouse
clone C3-33, ascites fluid
| MDL number: | MFCD00282461 |
Description
| Immunogen | dog cardiac ryanodine receptors (RyR) |
| Specificity | The antibody reacts strongly with RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform), and weakly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform). It reacts with ryanodine receptor in cardiac muscle of canine, rat, finch and pigeon; in visceral smooth muscle of toad; and in rat brain. It reacts with skeletal muscle of fish and the β isoform of frog, but only weakly with skeletal muscle in rabbit and the α isoform of frog. In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in the hippocampus. |
| Physical form | Diluted ascites fluid containing 0.05% sodium azide |
Properties
| antibody form | ascites fluid |
| clone | C3-33 (monoclonal) |
| mol wt | antigen mol wt ~565 kDa (non-mammalian vertebrates, a doublet at 565 kDa represents the α and β isoforms) |
| species reactivity | pigeon, canine, rat, fish, rabbit (weakly), finch, frog, toad |
| concentration | ~1 mg/mL |
| application(s) | immunoblotting (chemiluminescent): 1 μg/mL |
| immunohistochemistry (frozen sections): 1 μg/mL using 4% paraformaldehyde-fixed, frozen mouse brain tissue | |
| immunoprecipitation: suitable | |
| isotype | IgG1 |
| Gene Information | rat ... Ryr1(114207), Ryr2(84025) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
References
| reference | Lai, F.A., et al., Amphibian ryanodine receptor isoforms are related to those of mammalian skeletal or cardiac muscle. Am. J. Physiol. 263, C365-C372, (1992) Abstract |
| Brutkiewicz, R., et al., The VSV Matrix Protein Impairs CD1d-mediated Antigen Presentation through Activation of the p38 MAPK Pathway. J. Virol. 64, 1142-1157, (2008) |
